Biodesulfurization is a biotechnological course of that employs microorganisms as biocatalysts to take away sulfur from fuels normally at mesophilic circumstances, concentrating on recalcitrant organosulfur compounds with out affecting their hydrocarbon construction. One of many bottlenecks hindering its large-scale software is the inhibition of biodesulfurization exercise by simply metabolized sulfur compounds, comparable to sulfates, even at residual concentrations. This will increase manufacturing prices by requiring high-purity sulfur-free vitamins or complicated induction steps to forestall/revert inhibition. The target of this work was to bypass this limitation and exhibit that it’s doable to provide biocatalysts with biodesulfurization exercise utilizing sulfate as the one sulfur supply, with out using inducers or genetic manipulation, just by adjusting the sulfur:carbon ratio in steady tradition. With this objective, the bacterium Gordonia alkanivorans pressure 1B was cultivated in a chemostat with a medium containing 10 g/L of fructose as carbon supply and completely different sulfate concentrations (12-50 mg/L SO42-) utilizing Na2SO4. Then the micro organism have been employed as biocatalysts in biodesulfurization assays with a recalcitrant organosulfur compound (dibenzothiophene). Underneath these circumstances it was noticed that 2.2 mgsulfate/gfructose ensured a biodesulfurization exercise of 6.1 µmol/gDCW/h, 15% better than beforehand reported for this pressure with an inducer, with out limiting biocatalyst manufacturing. This novel process was additional utilized to a different biocatalyst, Rhodococcus erythropolis pressure D1, validating its vast applicability to different desulfurizing microorganisms. General, these outcomes point out a beforehand unknown regulation mechanism depending on relative sulfur focus, which influences mobile responses and regulates biodesulfurization exercise, permitting using simply metabolized sulfur sources to provide cost-effective biocatalysts for biodesulfurization.