AMG-1/SLRP-1 is required for spermatogenesis in C. elegans: Examine

The mechanisms of RNA-binding proteins (RBPs)-mediated post-transcriptional regulation of pre-existing mRNAs, which is important for spermatogenesis, stay poorly understood.

On this research, researchers determine {that a} germline-specific mitochondrial RBP AMG-1(irregular mitochondria in germline 1), a homolog of mammalian leucine-rich PPR motif-containing protein (LRPPRC), is required for spermatogenesis in Caenorhabditis elegans.

The amg-1 mutation hinders germline growth with out affecting somatic growth and results in the aberrant mitochondrial morphology and construction related to mitochondrial dysfunctions particularly within the germline. Researchers reveal that AMG-1 is most continuously certain to mtDNA-encoded 12S and 16S ribosomal RNA, the important elements of mitochondrial ribosomes, and that 12S rRNA expression mediated by AMG-1 is essential for germline mitochondrial protein homeostasis.

Moreover, steroid receptor RNA activator (SRA) stem loop interacting RNA binding protein (SLRP-1), a homolog of mammalian SRA stem loop interacting RNA binding protein (SLIRP) in C. elegans, interacts with AMG-1 genetically to control germline growth and reproductive success in C. elegans. General, these findings reveal the novel operate of mtRBP, particularly in regulating germline growth.

This research was led by Prof. Lengthy Miao (Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Training, School of Life Science, Beijing Regular College). The paper is revealed within the journal Science Bulletin.