DLC pretreatment is a novel and environment friendly pretreatment methodology for lignocellulosic biomass, however its mechanism has not been illustrated but. This research performed a scientific investigation and located that the synergy of acidic chemical motion and mechanical motion throughout DLC pretreatment performed an necessary position for wonderful efficiency. The mechanical actions seemingly uncovered the inner polysaccharides of biomass by disrupting the cell wall construction, and the uncovered polysaccharides reacted with concentrated acid and have been partially deconstructed, thereby considerably lowering the severity of subsequent dilute acid remedy. This synergism elevated the accessible breakpoints of crystalline cellulose to cellulase by lowering the cellulose crystallinity and polymerization diploma in addition to digesting amorphous cellulose. Moreover, the big elimination (about 60%) of hemicellulose elevated biomass porosity. The native lignin construction was properly preserved with out technology of pseudo-lignin and enormous quantities of poisonous degradation merchandise. These properties contribute to the excessive sugar restoration (97.2%), nice digestibility (83.4% whole monosaccharides yield) and excellent fermentability of DLC biomass, which weren’t achievable for conventional dilute acid pretreatment. Simultaneous saccharification and co-fermentation of DLC pretreated rice straw resulted in an ethanol titer as excessive as 82.9 g/L with out detoxing. Detailed structural evaluation revealed that the well-preserved inter-unit linkages (over 80%) and non-condensed subunits remained in DLC lignin. This research revealed the mechanism of DLC expertise in selling biomass conversion, offering potential steerage for realizing environment friendly and economical biorefinery.