Microbial cell separation and recycling has grow to be the key cost-effective difficulty in business fermentation biotechnology, particularly the difficult-to-cultivate strains. On this regard, high-speed centrifugation is a big industrial operation for bacterial separation however at the price of high-end tools and vitality consumption. Due to this fact, the current research proposed a novel particle resin aiding technique for the primary time to facilitate the centrifugal separation and recycling of microbial cells from fermentation broths, which decreased the centrifuge pressure for 3 microbial cells by 26%~36%. which considerably diminished the centrifuge pressure of three consultant microbial cells, yeast, gram-positive micro organism and gram-negative micro organism, by 26% to 36%. Particularly, the resin aiding centrifugation efficiently obtain an environment friendly separation at 563g for the small dimension micro organism of Gluconobacter oxydan. By comparability with glass or metal particle, the mechanism of resin-assisting cell sedimentation was analyzed from the points of resin granularity, porosity, charged teams and the isoelectric level of bacterial cell. The interplay mannequin was due to this fact hypothesized for particle resin to microbial cells. As well as, the environment friendly separation was simply realized for cell reuse and resin restoration by a easy operation of recent fermentation medium injection. The resin aiding technique gives a easy and inexperienced technological method for the separation and recycle of small bacterial cells in fermentation and biotechnological business.