CONSTANS (CO), a well known member of the B-box household, is the key part of the photoperiodically regulated flowering and circadian pathways in Arabidopsis (Arabidopsis thaliana). CO is expressed in numerous tissues, together with seedling roots and younger leaves. Nevertheless, the roles and underlying mechanisms of CO in modulating physiological processes exterior of flowering stay unclear.
In a examine printed in Plant Physiology, researchers from the Xishuangbanna Tropical Botanical Backyard (XTBG) of the Chinese language Academy of Sciences confirmed that CONSTANS protein negatively modulated salinity tolerance in Arabidopsis by bodily associating with and antagonizing the abscisic acid-responsive component binding issue (ABF) transcription elements beneath long-day situations.
The researchers first analyzed the spatial sample of CO expression and detected its transcripts in younger leaves and seedling roots. They noticed that the CO transcription and proteins ranges decreased in salt-stressed seedlings.
They then carried out phenotypic analyses and located that CO negatively mediates salinity tolerance beneath lengthy day (LD) situations. The loss-of-function CO mutant seedlings have been extra tolerant to salinity stress, whereas over-expression of CO resulted in vegetation with decreased tolerance to salinity stress.
Genetic analyses revealed that the damaging function of GIGANTEA (GI) in mediating salinity tolerance required a practical CO protein. Additional investigations confirmed that CO bodily interacts with the Abscisic Acid-Responsive Aspect Binding Factor1 (ABF1), ABF2, ABF3, and ABF4, which belong to a big household of primary leucine zipper domains.
These ABFs have been positively concerned within the modulation of salinity tolerance. CO repressed the expression of a number of salinity-responsive genes and interfered with the transcriptional regulatory operate of ABF3.
“Our outcomes recommend that CO antagonistically interacts with ABFs to inhibit salinity responses. This examine offers a mechanistic understanding of how plant salinity tolerance is negatively regulated by CO functioning along with ABFs transcription elements,” stated Hu Yanru of XTBG.
Jiancan Du et al, CONSTANS interacts with and antagonizes ABF transcription elements throughout salt stress beneath long-day situations, Plant Physiology (2023). DOI: 10.1093/plphys/kiad370
Chinese language Academy of Sciences
CONSTANS protein negatively modulates salinity tolerance in arabidopsis, examine exhibits (2023, July 4)
retrieved 4 July 2023
This doc is topic to copyright. Other than any truthful dealing for the aim of personal examine or analysis, no
half could also be reproduced with out the written permission. The content material is supplied for data functions solely.