Diluted aqueous ionic liquid assists the acidic oxidative hydrolysis of water-soluble recalcitrant polysaccharide xanthan by structural deterioration

Pure or extremely concentrated ionic liquids (ILs, C_IL > 40% v/v) have been broadly utilized for the preparation of value-added merchandise from polysaccharides by breaking their hydrogen bonds. Decreasing the dosage of ILs would profit the institution of a greener course of. Nevertheless, the impact of low-concentration ILs on the pretreatment of polysaccharides, particularly water-soluble recalcitrant polysaccharides, has not been absolutely investigated. On this research, aqueous [Bmim]Cl (1-butyl-3-methylimidazolium chloride) with completely different dilution ratios was utilized to deal with xanthan gum (XG) and help XG hydrolysis. Structural characterization and hydrolysate analyses confirmed that 1% (v/v) [Bmim]Cl may successfully break intramolecular hydrogen bonds of XG by the binding of [Bmim]⁺ to XG facet chains, disrupting the XG conformation. Such adjustments elevated the accessibility of XG for chemical reagents. Furthermore, [Bmim]Cl may considerably improve the content material of hydroxyl and superoxide radicals (from H₂O₂) within the response system. These interactions strengthened the acidic oxidative hydrolysis of XG. Notably, a low focus of [Bmim]Cl was proved to inhibit the Maillard browning of the acidic oxidative hydrolysis system, which additionally contributed to XG degradation. Beneath hydrolysis situations of 0.4 M HCl, 0.75% (w/v) H₂O₂ and 1% (v/v) [Bmim]Cl for 4 days at 80 °C, XG could possibly be hydrolyzed into a combination with a excessive dextrose equal worth of 29.16% containing 25 sorts of oligosaccharides. In the meantime, the XG hydrolysates confirmed good antioxidant means and elicitor exercise in opposition to soybean cotyledon. Our work needs to be helpful for establishing a inexperienced and financial platform for valorizing water-soluble recalcitrant polysaccharides.